Versatile low-copy-number plasmids for temperature-inducible overexpression of bacterial genes in Escherichia coli.

نویسندگان

  • C Götting
  • G Thierbach
  • A Pühler
  • J Kalinowski
چکیده

2.Bailey, J.E., N.A. Da Silva, S.W. Peretti, J.H. Seo and F. Srienc. 1986. Studies of hostplasmid interactions in recombinant microorganisms. Ann. NY Acad. Sci. 469:194-211. 3.Lerner, C.G. and C.D. Carter. 1995. PCR colony screening using the scintillation proximity assay to detect inserts in cloning vectors. BioTechniques 19:914-917. 4.Liu, Z. and N.C. Mishra. 1995. Single-tube method for plasmid miniprep from large numbers of clones for direct screening by size or restriction digestion. BioTechniques 18:214217. 5.Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.

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عنوان ژورنال:
  • BioTechniques

دوره 24 3  شماره 

صفحات  -

تاریخ انتشار 1998